The Macromolecular Structure Group is located in the University of Florida's Brain Institute.
 
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View of the lab
The lab has 1600 square feet of space. It consists of a general wet-lab area - with benches, a cold room, a Beckman centrifuge, a Beckman ultracentrifuge, two microfuges, spectrophotometer, two microscopes, two chemical hoods, freezer, fridge, water bath, PhastGel system, and a conferencing table. The lab. also contains a core computing/graphics room, two offices, a crystallization room, and a solution making room.

 
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X-ray machine
The in-house X-ray diffraction data collection facility (Rigaku generator, R-AXIS IV++ imaging plate system, osmic mirrors and an Oxford Cryosystems Cryostream).

www.msc.com

 
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Generator
The RU-300 provides up to 18 kW (60 kV,300 mA) of power output. Maximum power density of 12 kW/mm2 is available for a 0.1 X 1 mm focal spot size.

MSC Generators

 
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Image Plate
The R-AXIS IV++ is the latest imaging plate (IP) area detector from Rigaku, the global leader in IP technology for X-ray diffraction. Starting with the proven design of the R-AXIS IV, Rigaku has improved the experimental capabilities of the instrument by enhancing a number of key components including a new imaging plate, a higher powered laser, an improved controller, and a greater flexibility in image format.

 
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Osmics
Osmic Inc, a Rigaku Company, produces a multilayer optic called the Confocal Maxfluxä optic. In this optic product, two graded multilayer optics are glued together in a perpendicular orientation. The advantage of this configuration is that both optics are the same distance from the X-ray source and thus capture a larger view of the source resulting in a higher flux beam.

MSC Osmics

 
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Oxford Cryo-system
Nitrogen Gas Low Temperature Cryostat for X-ray Crystallography

Oxford Cryosystems

 
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Silicon Graphics Workstations
There is a well established core computing/graphics room within the laboratory space housing 3 Silicon Graphics Octane workstations, 2 O2 workstations and 3 Indy workstations. The primary use of these computers is structure determination calculations, structure refinement and molecular graphics modeling. There are also 5 PC computers and a MacIntosh Powerbook. All the computers have the appropriate software packages required for structural biology and internet access to various databases.
 
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Protein Purification System
Pharmacia Akta FPLC.
 
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Crystallisation Room
This is where we grow our crystals for structural studies. Note the back wall showing our "crystal wall of fame".
 
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Electron Microscope Facility
The Joel JEM-100CX II electron microscope is used for the routine checking of negativity stain virus preps prior to cryo-freezing or crystallization.
 
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Cryo Plunger
The plunger is used to flash freeze samples for cryo-Electron Microscopy structural studies.
 
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Tissue Culture Facilities
This 400 square feet of space is a dedicated share facility for virus propagation. It has 4 virus hoods, 3 water-cooled CO2 incubators, an upright cooler, 2 centrifugation units, and a refrigerator. The facility also has a small adjoining room with a light microscope.
 
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Spectrophotometer
Beckman DU 530
 
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Water Purification System
U.S. Filter


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