Structural Studies of Plant Geminiviruses:
Carolina G. Casado, Javier Ortiz, Mavis Agbandje-McKenna
Biohysical methodologies, such as X-ray crystallography and cryo-electron microscopy (Cryo-EM) combined with X-ray crystallography, enable the atomic visualization of interaction interfaces between protein subunits and between proteins and nucleic acids in macromolecular aseemblages, including viruses. These studies have demostrated the requirement for a high degree of fidelity in the interactions between components, within a precise geometric constraint, in the steps that precede and result in the assembly of mature infectious virus particles. They also suggest the possibility for viral control based on assembly disruption. However, the extent to which the available information applies to the unique twined icosahedral virus capsid arrangment of the geminiviruses is unkown. This project aims to fill this dearth in our knowledge base. The model virus to be exploited is the well characterized Nigerian strain of the plant geminivirus maize streak virus (MSV-N). The nature of the MSV coat protein, the geometric components in the MSV viral capsid assembly pathway and the essential protein-protein and protein-DNA interactions that are required for building the unique geminate capsid are examined by biochemical and structural (X-ray crystallography and cryo-EM) methods. These studies should validate the computational models to be developed for probing the driving force behind the MSV geminate capsid assembly.
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